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Flies were then washed (twice for 1 min each) with 0.15 M sodium cacodylate buffer pH 7.4 containing 2 mM CaCl 2 on ice and were reacted with 2.5 mM Ce-DAB2 solution in cacodylate buffer 36 for 75 min to produce the desired brown intensity color from the precipitate

The asarone in VOA is found to be principally responsible for the toxic effects of VOA

During the time-restricted eating (TRE) (or time restricted feeding -TRF- in animal models) daily eating hours are reduced (Liu et al., 2022), allowing food intake ad libitum in windows ranging from 4 up to 10 h (1420 h of fasting per day) (Lowe et al., 2020