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After three PBS washes, iHLOs were incubated in 100 L Ce3D permeabilization/blocking buffer (BioLegend, catalog: 427706) for 1 h at room temperature

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Results: No significant baseline differences were found among the three thrombus groups: F/P-rich, RBC-rich, and F/PRBC, in terms of age (median: 70, 63 and 72 years), sex (male: 63%, 81% and 61%), NIHSS score (median: 17, 17 and 16), intravenous alteplase use (54%, 38% and 72%), or ASPECTS score (median: 8, 9 and 9)