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Following the sgRNA library transduction at a low MOI (MOI 0.3), cells were exposed to doxycycline to induce Cas9 expression and gene mutagenesis, and then exposed to vehicle (DMSO) or palbociclib 500 nM (SF 50 ) for two weeks

In contrast, the combination of BSO and AUR significantly reduced cell viability in all 3 cell lines compared to the other treatment groups ( Figure 2A )