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Se-catalysed standard reaction conditions N -phenoxyamides ( 1 ) (0.20 mmol), PhSeBr (10 mol%), were weighed into a 10 mL tube, to which was added 1,4-dioxane (2.0 mL)

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The Mito-Grx1-roGFP2 construct (Addgene, 64977) was cloned into the pLenti-CMV-Puro vector (Addgene, 17448) and lentivirus generated to produce stable cell lines expressing the biosensor as described