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Since the enzyme glutathione reductase, which reverts free glutathione from its oxidised form (GSSG) is constitutively active and inducible upon oxidative stress, free glutathione exists almost exclusively in its reduced form

Then, they calculated the network contribution index through mathematical formulas to determine the core components

10.12669/pjms.40.11.10317 141 LinX.ChenH.XieY.ZhouX.WangY.ZhouJ.et al (2022)

A similar dietary supplementation was also found to decrease the phosphorylation of PPAR and nuclear factor E2-related factor 2 (Nrf2) and induce upregulation of their downstream targets including -glutathione S-transferase (GST) and adenosine triphosphate-binding cassette transporter A1 (ABCA1) through inhibition of matrix metalloproteinase-1 (MMP-1) (Bozaykut et al., 2014)