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Moreover, GPX4, SLC7 A11, and GSH were decreased, indicating a compromised GPX4/GSH system

The parasite pellet (200 l) was mixed with 150 g of purified plasmid (pARL-1a+ hGrx1-roGFP2) in 400 l of cytomix and then electroporated at 0.310 kV and 950 F (Gene pulser, Bio-Rad) as described [34]

Blots were incubated overnight at 4 C with HRP-conjugated anti-nitrotyrosine mouse mAb (1:2500 dilution in CanGetSignal solution 1,NKB-101, Toyobo, Osaka, Japan), followed by ECL-detection on a FUSION SOLO.7S.EDGE imaging system (Vilber Lourmat, Marne-la-Valle, France)

However, to maximize their therapeutic potential and overcome current limitations, several future directions should be considered