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For studies with a calorie-restricted and body-weight-loss-matched control group, HFHS diet (58 kcal% fat, D12331i, Research Diets) was weighed and provided as one separate pellet per mouse at the time of injection

It has been demonstrated that arginine and leucine prompt mTORC1 by activating RAS-related GTPase (RAG) complex

This, may in part, be improved by genetically engineering the probiotics involved

In contrast, iron overload induced significant downregulation of ACSL4 and GPX4 expression in these cells, suggesting a disruption in lipid homeostasis and a reduction in antioxidant defense mechanisms, which may contribute to the progression of iron-induced ferroptosis