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Autophagy can also maintain healthy stem cell niches

reuteri NK33 can activate microglial cell infiltration into the hippocampus and induce hippocampal brain-derived neurotrophic factor (BDNF) expression and cAMP-response element binding protein (CREB) phosphorylation in IS-exposed mice as well as LPS-stimulated SH-SY5Y cells by suppressing the activation of the NF-B pathway and HPA axis, thus suggesting that NK33 alleviated the suppression of NF-B-mediated BDNF expression in the hippocampus, with the regulation of LPS infiltration into the brain, leading to the attenuation of anxiety and depression

Western blotting RIPA lysate was used for cellular protein extraction

(12) D Philp, M Nguyen, B Scheremeta, et al (2004) Thymosin beta4 increases hair growth by activation of hair follicle stem cells FASEB Journal, 2004 Feb, Volume 18 (Issue 2), Pages 385-7