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SCO1, SCO2, and COX11 are used to direct copper transport to various metalloenzymes and copper-dependent ATPases with copper output and metal chaperone functions, including ATP7A and ATP7B

For GSSG quantification, the GR-based assay was performed on the neutralized extract following incubation with 2-vinylpyridine as described by The floret concentrations of non-phosphorylated and phosphorylated pyridine dinucleotides were determined with a recycling assay that detected the phenazine methosulfate-based reduction of dichlorophenolindophenol as described by + ) and nicotinamide adenine dinucleotide phosphate (NADP + ) determinations, 100 mg of frozen floret tissue was combined with 1 mL of 0.2 N HCl, vortexed for 5 min and centrifuged at 13,000 g and 4C for 10 min

In the animal study, livers were collected from wild-type (WT), Fgf15 transgenic (TG), and Fgf15 knockout (KO) mice every 4 hrs over a 24-hr period

Detcheverry et al