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The upper layer was collected, and absorbance was recorded at 520 nm using toluene as a blank

On the other hand, in hypoxia, PDHs and VHL are inactivated by mild oxidative stress, and HIF-1 is released from PHDs and VHL, transferred into nuclei, where HIF-1 forms a heterodimer with HIF-1, and results in the transcription of hypoxia response elements (HREs) on DNA

Gently shake to ensure the complete the solubilization, and then administer the solution thus obtained by slow intravenous route.

Thiol and disulfide metabolites of the radiation protector and potential chemopreventive agent WR-2721 are linked to both its anti-cytotoxic and anti-mutagenic mechanisms of action