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Description

Cell pellets bearing GST-GATE16 were resuspended in 50 ml buffer PBS (140 mM NaCl, 2.7 mM KCl, 10 mM Na 2 HPO 4 , 1.8 mM KH 2 PO 4 , pH 7.3), dounced until homogeneous, and sonicated using Qsonica Sonicator (5 s on, 10 s off for 5 min, amplitude 35%)