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4,5 Deficient GSH synthesis has been associated with oxidative stress in aging, 6 and GSH concentrations decrease with age, a factor thought to explain, in part, why the elderly are at greater risk of neurodegenerative diseases

GSH is synthesized in two steps: First, gamma-glutamylcysteine (-GC) is formed from glutamate and cysteine, which is the rate-limiting step in GSH production

Amino acids W554, M1094, W1247 and R1250 were observed to spatially restrict the movement of GSH into the direction of the cytosol, while ritonavir blocks the movement towards the P-pocket, thereby preventing the diffusion of GSH out of the binding site