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Cultures were passaged by trypsinization (0.05% trypsin-0.53 mM EDTA) every week and were seeded at a density of 1.2 10 4 cells/cm 2 in 60 20 mm or 100 20 mm diameter culture dishes (Sarsted, Nmbrecht, Germany), 96-well or 24-well plates (Sarstedt), or 15 -slide 4-well coverslip (Ibidi USA, ThermoFisher Scientific) for subsequent experiments

Macrophages within granulomas are able to convert more active vitamin D, leading to increased calcium absorption from the digestive tract

Furthermore, GPX2 functions as a temporal gatekeeper of cell fate decisions during endoderm differentiation, with distinct stage-specific roles

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