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4 Endogenous GHK improves tissue repair in the skin and possesses cell protective activity

The nasal mucosa is highly vascularized, allowing absorption directly into circulation, and some evidence suggests nasal administration may provide more direct central nervous system access via the olfactory pathway

RNA isolation and quantitative RT-PCR Total RNA from cells was isolated with Trizol reagent (Qiagen GmbH, Hilden, Germany) and RT-PCR was conducted using a quantitative SYBR Green RT-PCR kit (Applied Biosystems, Warrington, UK) was used with a Step One Plus RT-PCR System (Applied Biosystems)