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Each well was then washed with a washing buffer solution provided by the company and 50 L of streptavidin-HRP solution was added to each well and the plate incubated at room temperature for 30 min with gentle shaking on a plate shaker

Wang and Brautigan described that the M105I mutation provokes a gain of function in the AMPK phosphatase activity of -SNAP 7 , strongly suggesting that the M105I mutation affects some SNARE-independent protein-protein interaction-dependent functions

The mobile phase consisted of (A) 0.1% aqueous phosphoric acid and (B) acetonitrile