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PVT, paraventricular nucleus of the thalamus

Clearly, some kind of selectivity must be exercised if complex mixtures are to be avoided

A marked inter-patient variability in purinergic metabolites emerged as a consistent feature of the cohort

In particular, DNA samples (50 ng) were amplified using the respective LightSNiP Assay for each SNP and Lightcycler FastStart DNA Master HybProbe Mix (Roche, Germany), according to the following PCR protocol: initial denaturation for 10 min at 95C, followed by 45 cycles of denaturation for 10 s at 95C, primer annealing for 10 s at 60C and extension for 15 s at 72C, followed by melting curve analysis to determine homozygosity for the wild-type alleles, heterozygosity, and homozygosity for the variant alleles