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A density of 5 10^5 cells per well in 500 l medium was plated in 24-well culture plates and incubated at 37C with 5% CO

The quantity of evaporation is not typically targeted for water efficiency, as it is responsible for the cooling effect. Source: EPA: Water Efficiency Management Guide Mechanical Systems, Nov 2017 Drift, leaks and overflow While evaporation is considered a necessary and acceptable cause of water loss, other means of water loss must be carefully controlled

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