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doi: 10.1016/j.freeradbiomed.2004.01.001 139 YoonJSLeeHJChoiSHChangEJLeeSYLeeEJ

D.ManzinoL.SonsallaP

After putting the finished reaction mixture in a thermal cycler, the following procedure was run: reverse transcription for 30 min at 50 C, primary denaturation for 10 min at 94 C, 40 cycles of 94 C for 15 s, annealing temperatures for 1 min as indicated in Table 5, and 72 C for 30 s