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Western blotting To analyse protein levels, cells were lysed with RIPA buffer (Biyuntian, P0013K)

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We also include a full B-complex, including methyl B12 and methyl folate given their critical role in brain and nervous system function

100 g/L) were prepared for the quantification, the first being ultrapure (18.2 M*cm) water, the others were diluted from a multielement standard solution (CPA-Chem, 33 elements, each 1000 mg/L in 2% nitric acid), each point containing 1 mL of ultrapure 67% nitric acid in a total volume of 50 mL