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World J Gastroenterol (2015) 21(5):145767

Thereafter, the pH was adjusted to between 5.0 and 6.0 with 100 L of 0.2 N NaOH and 40 L of 0.2 M NaH 2 PO 4 (pH 5.6)

In particular, the latter was completely absorbed by internal cells, resulting in a more uniform fluorescence distribution signal throughout the whole cell sphere