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Description

The membrane was treated with ECL prime blocking solutions (GE Healthcare, Little Chalfont, UK) and reacted overnight at 4 C with each antibody (Table S1) diluted in blocking buffer

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For GSSG measurement, 2-vinylpyridine (Sigma-Aldrich) was added before the enzymatic reactions to remove reduced glutathione

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A regular enema primarily aims to relieve constipation

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doi: 10.1023/a:1012827221844 64 ChenZ-SGuoYBelinskyMGKotovaEKruhGD

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