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RNA isolation, quantitative PCR, and RNA-sequencing Total RNA was extracted using an RNeasy Mini Kit (Qiagen, 74104)

10.1038/ncomms10845 43

Lancet 397 , 15771590 (2021)

Rhbdd3 further recruits the deubiquitin enzyme A20 through the K27-linked polyubiquitin chain on Lys268, inhibiting K63-linked NEMO polyubiquitination