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For detection of metabolites associated with methylglyoxal, targeted selected ion monitoring (tSIM) scans in positive mode were included

Minhas PS, Jones JR, Latif-Hernandez A, Sugiura Y, Durairaj AS, Wang Q, et al

Proc Natl Acad Sci 97:86588663

Another study indicates that TMAO enhances the synthesis of SMAD3, a crucial regulator of fibrosis, and elevates the likelihood of atherosclerosis and thrombosis, hence increasing the risk of ischemic heart disease (74)