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Unlike fat-soluble vitamins (A, D, E, and K) which are stored in adipose tissue and the liver for extended periods, water-soluble vitamins generally dissolve in water and are not stored in large quantities

Drug Design, Development and Therapy 9:2485-2499

This suggests an intrinsic degradation pathway independent of withdrawal frequency, though the magnitude is small enough that the samples remained above the literature-supported effectiveness floor

Therapies that rely on scaffold-free cellular approaches, such as ACI, are not designed to handle these mechanical burdens early post-surgery, and thus, utilizing scaffolds with improved bulk mechanical properties could increase efficacy of treatment and promote earlier return to normal activity in these knees