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NNMT inhibition and metabolic reprogramming Nicotinamide N-methyltransferase (NNMT) is an enzyme that becomes overexpressed in adipose tissue during obesity

Scientific Research HCG France Human Chorionic Gonadotropin (HCG) peptide has seen many advances in its potential medical applications, particularly in reproductive therapies

For Mitochondrial membrane potential (MMP) analysis, the cells were stained by 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolocarbocyanine iodide (JC-1) (5 g/mL) for 20 min and then washed with PBS for three times

Cloning, protein expression and purification N terminal His 6 -tagged hsDDB1 BPB (UniProt: Q16531, aa396705 replaced with a GNGNSG linker) and FLAG-tagged hsCRBN (UniProt: Q96SW2) were cloned into pAC8-derived vectors 133