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EGCG-Zn treatment significantly downregulated the expression of multiple key proinflammatory genes in the MAPK pathway (such as Fos, Jun, and Mapk8), suggesting that it could alleviate radiation-induced tissue injury by inhibiting MAPK-mediated inflammatory amplification and apoptotic signaling

Centrifuge at 10,000 x g for 2 minutes (or sufficient to produce a clear supernatant) Collect the supernatant and aliquot an appropriate portion for protein analysis

As we seek to develop more potent PINK1 inhibitors based on these scaffolds, the challenge will be to reduce the potency against other kinases to achieve sufficient selectivity for their use as chemical probes

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