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The persistent inflammatory response in the lungs is recognized as a precursor to fibrosis

Q5 high-fidelity DNA polymerase (New England Biolabs, Ipswich, MA, USA) and specific primers were used to perform mutations, which were verified by DNA sequencing in ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA, USA)

Given that EtOH-induced mitochondrial dysfunction and ER stress are intimately involved in the pathogenesis of ALD, future studies to examine these pathways under the current experimental setting will provide important mechanistic information underlying the protection seen in GCLM KO mice

Heliyon 10 (15), e34916