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During every visit to the clinics, patients were requested to bring medication packages for tablet counting, as a proxy of adherence ( All study participants underwent a baseline evaluation, which included physical examination, sputum (spontaneous or induced whenever sputum production was considered insufficient) or tracheal aspirate in unconscious patients, CD4+ T-lymphocyte count, HIV viral load, serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), bilirubins, and screening of concomitant drug exposures and chest radiograph, as previously described ( Respiratory samples were submitted to smear ZiehlNielsen staining technique, Xpert-Mtb/RIF for Mtb, and sown in liquid culture BACTEC MGIT 960 and solid culture Lwenstein-Jensen as previously described ( Laboratory Measurements Serum samples were used to measure total oxidant status, total antioxidant status and glutathione levels

Edden, R