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2.3 Data analysis All data were analyzed in Osprey software package (version 2.4.0)(Oeltzschner et al., 2020), using consensus-recommended pre-processing(Choi et al., 2021) and linear-combination modeling

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A 2-way-ANOVA followed by Sidaks multiple comparison test assessed both NAC-treatment and IPTB-severity factors for each blood compartment and metabolite, while the non-parametric Kruskal-Wallis test was used to assess metabolite differences between blood compartments in each IPTB and treatment subgroup, followed by the Wilcoxon matched-pairs signed-rank test for multiple comparisons between maternal and paired-cord blood samples

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