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It is hypothesized that 13-cis RA may hinder cell proliferation, promote cell death, alter gene and protein expressions, and diminish cell survival pathways in human meibomian gland epithelial cells

J Biol Chem 281:1038910398

At the end of the experiments, the mice were sacrificed (cervical dislocation for short distribution time points and carbon dioxide asphyxia for longer ones), and the draining LNs (ipsilateral popliteal), the second LN in the chain (lumbar), and nondraining LNs (axillary and contralateral popliteal LNs) were rapidly dissected free (Figure 1A)