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For experiments in 6-well plates, 50,000 cells were seeded, and after 24 hours, the medium was aspirated and washed once with PBS, and the indicated medium was added

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But its clinical efficacy is not ideal and glioma resistance to TMZ is the most important reason for chemotherapy failure

However, if the UPR fails to resolve the excessive buildup of unfolded proteins, it activates signaling pathways that promote inflammation and apoptosis, potentially leading to neurodegenerative disorders