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Given that MTX is more effective against dividing cells because of its effect on the nucleotide synthesis, we also examined correlations between metabolites concentrations and cell proliferation rate, as this could be a confounding factor (proliferation curves for doubling time determination are in Supplemental Figure 5 )

The caspase inhibitor Z-VAD-FMK alleviates endotoxic shock via inducing macrophages necroptosis and promoting MDSCs-mediated inhibition of macrophages activation