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To improve the expression, solubilization, and purification of recombinant proteins, it is therefore advised to alter the culture conditions by switching the medium or the temperature, co-expressing chaperones, adding large hydrophilic fusion tags as well as adding sucrose, raffinose, glycine, betaine and sorbitol during growth for enhancing the recombinant protein expression, solubilization and purification [5, 6]

doi: 10.1016/j.actbio.2022.07.055 203 WangLTongLXiongZChenYZhangPGaoYet al

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GenBank AA308984 EST179790 Colon carcinoma (Caco-2) cell line I Homo sapiens cDNA 5' end similar to ribosomal protein L27a, mRNA sequence