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Am J Physiology-Renal Physiol 316(3):F409F413

Cycling, the practice of using peptides for a defined period and then taking a break, is standard practice in peptide protocols

This was to see how GcvA initiates transcription of the glycine biosensor by binding to the gcvBp promoter, how GcvR inhibits GcvA binding to the promoter and how glycine stops GcvR binding to GcvA