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[6]Current Patent Assignee: PROTEOSTASIS THERAPEUTICS - WO2016/105477, 2016, A1 Location in patent: Page/Page column 0139

[4] At the cellular level, free estradiol diffuses through cell membranes and binds to intracellular estrogen receptors and , which upon ligand activation dimerize and translocate to estrogen-response elements on nuclear DNA, modulating transcription of genes governing reproductive tract integrity, thermoregulation, bone metabolism, and vascular endothelial function

The resulting spectral changes allowed us to monitor the balance between reduced (roGFP red ) and oxidized (roGFP ox ) forms of the sensor via ratiometric fluorescence microscopy 24

Springer New York, New York, pp 149158