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The role of age-associated B cells in systemic lupus erythematosus

Samples were resolved on 420% SDS-PAGE gels, transferred to PVDF membranes, blocked with 5% milk/TBS-T (1 hour, RT), and probed with anti-FYN (1:1,000) and anti-FLT-1 (1:1,000) antibodies in TBS-T overnight at 4 C