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Sample Preparation Accurately weigh the glutathione sample, add deionized water, mix thoroughly to prepare a sample solution with a concentration of 25 ~ 800 g/mL, which serves as the analytical solution

Treatment of various tumor cell lines with curcumin consistently alters ROS levels, decreases mitochondrial membrane potential, and upregulates pro-apoptotic markers such as Bax and cytochrome c, while downregulating anti-apoptotic proteins (see Table 2)

Additionally, retinoic acid enhances the expression of IGFBP3, which promotes antiadipogenesis and apoptosis, thereby influencing lipid storage and adipocyte turnover