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Afterward, a mixture of methanol-saturated petroleum ether and petroleum ether-saturated methanol (1:1, by volume) was sequentially added to the total fat, followed by sequential addition of petroleum ether-saturated methanol and methanol-saturated petroleum ether mixture (6:1, by volume) after shaking and homogenization

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10.1002/mds.29184

These factors include heavy smoking by their mothers in the first year of their lives, early exposure to food or formula, exposure to allergens found inside the house such as dust mites and animal dander, allergic complications in parents, and high serum levels of IgE ( 1.2 Allergic rhinitis complications AR causes inflammation of the nasal membrane