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An in vivo study using mice intra peritoneally injected with iron oxide NPs caused histopathological changes such as sloughing, detachment of germ cells, and vacuolisation in seminiferous tubules of the testes (155)

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However, the apparent increase in NP hydrodynamic diameter is several times than that of the original NP size, meaning that other mechanisms can contribute to this increase (NP agglomeration due to their colloidal destabilization, molecular bridge formation by the PC, itself)

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