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Molecular mechanism of NR4A1/MDM2/P53 signaling pathway regulation inducing ferroptosis in renal tubular epithelial cells involved in the progression of renal ischemia-reperfusion injury

Afterwards, 150 L DMSO was added to each well to dissolve the crystals constituted by the living cells and the absorbance at 490 nm was tested to get the relative cell viability

Also, the proliferation assay revealed that GSTM3 overexpression in MIA PaCa-2 stable cell lines resulted in significantly abolished proliferation capacity compared to NC cells (Figure 3B)