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when AA 4 is replaced, it is replaced by an amino acid selected from the group consisting of Glu, Asn and Asp

Since mTOR is located downstream of AMPK and mTOR has been well reported to regulate muscle growth and metabolism 42,43 as well as the eIF4EBP3L as a gatekeeper of mTOR in muscle growth 44 , we next examined the effect of mTOR pathway by measuring the levels of mTOR and eif4ebp3l in the LMNA mutant transgenic fish with or without drug treatment

Non-solubilized material was removed by centrifugation for 10 min at 4500 g

PCRs were performed using qPCR mix according to the manufacturer's instructions in an ABI 7500 PCR system (Applied Biosystems, USA)