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Think in visible signals: movement, grooming, appetite steadiness, and a body condition that looks comfortable. DVM Voice: Clinical Vignette of When Skin Changes Point Deeper Than the Surface Case provided by Sarah Calvin, DVM Maverick, a 4-year-old Siamese cat, was brought in for hair loss across his lower abdomen and red, flaky skin lesions that had progressed over the previous month

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The membranes were enucleated using a sharp blade, transferred onto a sheet of parafilm in a humidified tray, and then quenched with 50 mM NH 4 Cl in PBS-C/M for 10 min at room temperature