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MTX-induced hepato-renal damage was assessed by serum toxicity biomarkers (AST, ALT, BUN, and creatinine), oxidative stress estimation (MDA, GSH, SOD, CAT, peroxidase, and glutathione reductase), interleukins profiling (TNF-, IL-1, IL-6, and IL-12), tissue histopathology and immunohistochemical (caspase-3 and NFkB) examination

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There were no significant difference of gender, treatment time of PEG-IFN to attain HBsAg loss ( p =0.744), consolidation treatment time of PEG-IFN ( p =0.658), serum ALT ( p =0.681), serum HBsAb( p =0.052), compensated cirrhosis ( p =0.064) at the baseline of follow-up and also the HBsAg level before PEG-IFN treatment ( p =0.979)