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SIRT1 overexpression alleviates noise-induced oxidative stress, synaptopathy, and neurite degeneration in hair cells To further investigate the role of SIRT1 in mitigating oxidative stress, we examined the levels of 3NT and 4HNE, which are markers of oxidative damage, in cochlear hair cells from the four groups: AAV-CTRL, AAV-SIRT1, AAV-CTRL + noise, and AAV-SIRT1 + noise

Injection temperature was 240 C with the interface set at 230 C and the ion source at 200 C