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To reduce reactions: Allow the solution to warm to room temperature for 5 to 10 minutes before injecting (cold solution stings more) Inject slowly over 5 to 10 seconds rather than pushing the plunger quickly Apply gentle pressure with a cotton ball after withdrawing the needle (do not rub) Consider using a higher concentration to reduce injection volume If injection site reactions persist , switching to a different anatomical site or adjusting your reconstitution concentration may help

The other 3 mice in each group were intravenously injected with pimonidazole hydrochloride (60 mg kg 1 ) (Hypoxyprobe-1 plus kit, Hypoxyprobe Inc.)

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If you need to give yourself a subcutaneous injection, your provider will teach you how to do so