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The purification process followed the same steps as previously described (Section 2.7), but on a larger scale, using a 30-liter IMAC (BPG) column, an 80-liter Sephadex G-25 column, a 2-liter SP Sepharose Fast Flow cation exchange chromatography column (Cytiva, USA), and a 45-liter Superdex 200 BPG column (GE Healthcare, USA) for SEC-HPLC

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Perilipin expression alone has been shown to be sufficient to confer PKA-mediated lipolysis in CHO cells that lack HSL (126)

That usually looks like: 200 pounds 1020 pounds 250 pounds 1225 pounds This early phase is often driven by reduced appetite, fewer cravings, and feeling full sooner