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8 mM of reducing agent DTT (Sigma) was then added to the samples and incubated in a thermomixer at 30C for 30 minutes, followed by the addition of 20 mM iodoacetamide (Sigma) for protein carboxylation with incubation at 30C in the dark for 30 minutes

H Jean, I think if your surgeon is not going to repair your tendon, a cortisone injeciton every 6-12 months would not be unreasonable

For example, taking riboflavin with a large meal has been shown to more than quadruple its absorption

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