[link] Jelinek, D., Flores, A., Uebelhoer, M., Pasque, V., Plath, K., Iruela-Arispe, M.L., Christofk, H.R., Lowry, W.E., and Coller, H.A., "Mapping Metabolism: Monitoring Lactate Dehydrogenase Activity Directly in Tissue", J Vis Exp (2018)
The fluorescence intensity changes were normalized to the baseline values (F/F0) to quantify the H 2 O 2 dynamics
Abbreviations AchE: Acetylcholinesterase ANOVA: Analysis of Variance BBB: BloodBrain Barrier BSA: Bovine Serum Albumin COMT: CatecholOmethyltransferase DA: Dopamine DJ: 1DaisukeJunko1 (PARK7) DTNB: 5,5Dithiobis(2nitrobenzoic acid) EE: Encapsulation Efficiency FTIR: FourierTransform Infrared Spectroscopy GSH: Glutathione H&E: Hematoxylin and Eosin IAEC: Institutional Animal Ethics Committee LB: Lewy Bodies LPO: Lipid Peroxidation MDA: Malondialdehyde MD: Molecular Dynamics MM: GBSAMolecular Mechanics Generalized Born Surface Area MMP3: Matrix Metalloproteinase3 NAC: Nacetylcysteine NBT: Nitroblue Tetrazolium OECD: Organisation for Economic Cooperation and Development OPLS: 4Optimized Potentials for Liquid Simulations 4 PDB: Protein Data Bank PD: Parkinsons Disease PDI: Polydispersity Index PL: ProteinLigand RT: Rotenone RMSD: Root Mean Square Deviation RMSF: Root Mean Square Fluctuation ROS: Reactive Oxygen Species SC: SCarboxymethylLCystine SCSLNs: SCarboxymethylLCystineLoaded Solid Lipid Nanoparticles SD: Standard Deviation SEM: Standard Error Mean / Scanning Electron Microscopy (based on context) SLNs: Solid Lipid Nanoparticles SOD: Superoxide Dismutase TEM: Transmission Electron Microscopy UV: Ultraviolet References Feigin, V
Endothelial dysfunction is induced by the detrimental actions of high glucose levels and increased oxidative stress on endothelial cells that make up the vascular lining
