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Despite this extra CBM loop, the absence of a loop ~15 residues in length that resides at the Loop 1 position may explain why Bu GUS, Bd GUS, and other non-L1 GUS enzymes exhibited higher K m values for p NPG compared to Ec GUS, Ee GUS, Sa GUS, and Cp GUS

Purification of the crude by semi-preparative HPLC (eluent A: 0.1% TFA in water, eluent B: 0.1% TFA in CH 3 CN/water 4/1 v/v, 6 mL min 1 , 035% eluent B in 25 min, C18 column, detection at 215 nm, 30 C) furnished SetCys-ALKEPVHGA-MPA peptide 8a as a white solid after lyophilization (25.3 mg, 20%)

Again, the number of silique in D2TG plants grown in oxidative stress was almost two times higher than any of the plants

Kretschmer, B