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Phospholipase D (PLD) catalyzes the hydrolysis of the phosphate group from phosphatidylcholine, yielding diacylglycerol (DAG) and phosphatidic acid

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inhaled), (iv) the composition of the antioxidant agent itself ( i.e ., solubility, adjuvants, and additives), (v) selection of un-standardized, un-validated, or otherwise soft primary outcome indicators, including outcomes of limited relevance to the mechanism of antioxidant action, and (vi) lack of surrogate markers of oxidative stress to accompany functional outcomes

In tolerance assay, at 42 h after the end of conditioning regimen, shorter preconvulsive latencies than in healthy (non-diazepam conditioned) mice following isoniazid (800 mg/kg i.p.) (as hallmark of tolerance) were observed if diazepam (5.0 mg/kg i.p.) was again given acutely to mice previously conditioned with diazepam alone (use of picrotoxin 3.0 mg/kg i.p., as convulsant, with acute application of diazepam in previously diazepam conditioned mice did not lead to tolerance hallmark)